pivlab package  (MathWorks Inc)

Journal: STAR Protocols

Article Title: Protocol for characterizing airway epithelial ciliary beating and mucociliary transport using image processing and particle imaging velocimetry

doi: 10.1016/j.xpro.2025.103674

Figure Lengend Snippet: MCT Analysis and Results (A) Overall workflow for MCT analysis. (B) Snapshots showing representative original MCT and the corresponding image after stationary particles were removed. The scale bar represents 100 μm. (C) A screenshot showing the GUI of the PIVLab Matlab package after running. (D) Snapshots showing important settings to input into the PIVLab GUI necessary for MCT analysis. (E) Snapshot showing representative results obtained from MCT analysis of differentiated hSAEpCs in a TWI. The velocity vector field is indicated by the arrows while its magnitude is shown by colors with values presented by the color bar. (F) A plot showing the histogram of MCT velocity obtained from the analysis in (E).

Article Snippet: The scale bar represents 100 μm. (C) A screenshot showing the GUI of the PIVLab Matlab package after running. (D) Snapshots showing important settings to input into the PIVLab GUI necessary for MCT analysis. (E) Snapshot showing representative results obtained from MCT analysis of differentiated hSAEpCs in a TWI.

Techniques: Plasmid Preparation

Journal: STAR Protocols

Article Title: Protocol for characterizing airway epithelial ciliary beating and mucociliary transport using image processing and particle imaging velocimetry

doi: 10.1016/j.xpro.2025.103674

Figure Lengend Snippet:

Article Snippet: The scale bar represents 100 μm. (C) A screenshot showing the GUI of the PIVLab Matlab package after running. (D) Snapshots showing important settings to input into the PIVLab GUI necessary for MCT analysis. (E) Snapshot showing representative results obtained from MCT analysis of differentiated hSAEpCs in a TWI.

Techniques: Recombinant, Software, Microscopy

Journal: STAR Protocols

Article Title: Protocol for characterizing airway epithelial ciliary beating and mucociliary transport using image processing and particle imaging velocimetry

doi: 10.1016/j.xpro.2025.103674

Figure Lengend Snippet: CBF, CCP Analysis and Results (A) Snapshot showing the contents of the “info.xlsx” Microsoft Excel file. (B) Screenshot showing the commands and the corresponding input parameters to input into MATLAB when running the CBF, CCP analysis code. (C) A representative result of CBF, CCP analysis for the negative control that uses a blank TWI to determine background. (D) A representative result of CBF, CCP analysis for a TWI containing differentiated primary hSAEpCs. The heat map scales in C and D indicate beating frequency in Hertz. (E) Plot showing a representative CBF histogram obtained from the data in D and processed using GraphPad Prism 9. (F) Table summarizing several representative statistical parameters that can be obtained from analyzed CBF data using Descriptive Statistics tool from GraphPad Prism 9, to further characterize beating behaviors of cilia.

Article Snippet: PIVlab v.3.02 MATLAB packages , PIVlab , Link download.

Techniques: Negative Control

Journal: STAR Protocols

Article Title: Protocol for characterizing airway epithelial ciliary beating and mucociliary transport using image processing and particle imaging velocimetry

doi: 10.1016/j.xpro.2025.103674

Figure Lengend Snippet: MCT Analysis and Results (A) Overall workflow for MCT analysis. (B) Snapshots showing representative original MCT and the corresponding image after stationary particles were removed. The scale bar represents 100 μm. (C) A screenshot showing the GUI of the PIVLab Matlab package after running. (D) Snapshots showing important settings to input into the PIVLab GUI necessary for MCT analysis. (E) Snapshot showing representative results obtained from MCT analysis of differentiated hSAEpCs in a TWI. The velocity vector field is indicated by the arrows while its magnitude is shown by colors with values presented by the color bar. (F) A plot showing the histogram of MCT velocity obtained from the analysis in (E).

Article Snippet: PIVlab v.3.02 MATLAB packages , PIVlab , Link download.

Techniques: Plasmid Preparation

Journal: STAR Protocols

Article Title: Protocol for characterizing airway epithelial ciliary beating and mucociliary transport using image processing and particle imaging velocimetry

doi: 10.1016/j.xpro.2025.103674

Figure Lengend Snippet:

Article Snippet: PIVlab v.3.02 MATLAB packages , PIVlab , Link download.

Techniques: Recombinant, Software, Microscopy

Journal: Nature Communications

Article Title: Periodic splay Fréedericksz transitions in a ferroelectric nematic

doi: 10.1038/s41467-025-55827-9

Figure Lengend Snippet: a PolScope Microimager texture of the in-plane splay and bend for the normal incidence of light; wavelength 535 nm. b Polarizing microscopy of square lattice of +1/−1 defects. c Potential difference measured at the N F cell electrodes; the generated voltage. d Transmitted intensity as a function of time at locations L1’–L4’. \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${{\bf{P}}}$$\end{document} P oscillates with the frequency of the applied field. Incident laser beam makes an angle \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\beta=$$\end{document} β = 15° with the normal \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hat{{{\bf{z}}}}$$\end{document} z ^ to the cell. Dashed line corresponds to zero voltage. In ( a – d ), \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$d$$\end{document} d = (3.0 ± 0.1) μm; applied voltage from the source U rms = 30 V, 200 kHz sinusoidal waveform; 120 °C. e Particle image velocimetry (PIVlab, Matlab) integrated trajectories of fluorescent spherical flow tracers of the diameter 300 nm in the square lattice of +1/−1 defects; d = (4.8 ± 0.1) μm cell; sinusoidal wave of voltage 4.5 V and frequency f = 200 kHz; 115 °C. f the same cell, in-plane velocity field of the tracers.

Article Snippet: Their trajectories are uncovered by particle image velocimetry package PIVLab in Matlab.

Techniques: Microscopy, Generated